Chitosan, derived from chitin by partial N-deacetylation, has been found to be non-toxic, biocompatible and biodegrable. So chitosan has high economic value and environmental impact. The study mainly compared the antifungal activities with two types of chitosan (W-CS, I-CS). The results indicated that W-CS and I-CS had high antifungal activities against the pathogens caused camellia anthracnose. As W-CS and I-CS concentrations increased from 1 mg-mL -1 to 10 mg-mL -1 antifungal went up significantly. The optimal pH range of chitosan for the highest antifungal activity was 5.3 to 6.0 with tartaric and acetic acid as solvent. The recommend treatment of 2.5 mg-mL -1 W-CS+1%HAC had the inhibition rate of 72.6%, which had almost 500 times effectives than that of thiophanate. Our results should help to develop for a new type of environmental friendly pesticide.