A Routing Protocol is a rule that determines how routers can communicate with each other. The development of modern computer networks such as the Internet makes routing protocols increasingly required to find the best and efficient route. This study examined the performance of routing protocols RIPng, OSPFv3, and EIGRP in an IPv6 network by using GNS3 based on parameters of throughput, jitter and packet loss. Throughput is the actual speed of a network, the higher the throughput then the faster the data transfer can be completed. Jitter is the difference in the arrival time interval between packets at the destination node. To get a better value of QoS (Quality of Service), the value of jitter must be kept to a minimum. Packet loss is the number of lost data packets during the data transmission process. To get a better QoS value, the value of packet loss must also be kept to a minimum. The results of this research indicate that the RIPng routing protocol has the highest throughput value, whilst a combination of OSPFv3-EIGRP routing protocols has the smallest jitter value. Lastly, the RIPng routing protocol has the smallest value for packet loss.