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Publication . Preprint . 2020

In Vivo Transcriptomic Profiling using Cell Encapsulation Identifies Effector Pathways of Systemic Aging

Omid Mashinchian; Xiaotong Hong; Eugenia Migliavacca; Christophe Boss; Filippo De Franceschi; Joris Michaud; Emmeran Le Moal; +9 Authors
Open Access  
Published: 09 Mar 2020
Publisher: Cold Spring Harbor Laboratory
Abstract
AbstractSustained exposure to a young systemic environment rejuvenates aged organisms and promotes cellular function. However, due to the intrinsic complexity of tissues it remains challenging to pinpoint niche-independent effects of circulating factors on specific cell populations. Here we describe a method for the encapsulation of human and mouse skeletal muscle progenitors in diffusible polyethersulfone hollow fiber capsules that can be used to profile systemic aging in vivo independent of heterogeneous short-range tissue interactions. We observed that circulating long-range signaling factors in the old systemic environment lead to an activation of Myc and E2F transcription factors, induce senescence and suppress myogenic differentiation. Importantly, in vitro profiling using young and old serum in 2D culture does not capture all pathways deregulated in encapsulated cells in aged mice. Thus, in vivo transcriptomic profiling using cell encapsulation allows for the characterization of effector pathways of systemic aging with unparalleled accuracy.
Subjects by Vocabulary

Microsoft Academic Graph classification: Biology Skeletal muscle medicine.anatomical_structure medicine Cell biology Heterochrony Cell encapsulation Inflammation medicine.symptom Transcriptome In vivo E2F Transcription Factors Progenitor cell