Promoter architecture of mouse olfactory receptor genes
Copyright policy )Promoter architecture of mouse olfactory receptor genes
Odorous chemicals are detected by the mouse main olfactory epithelium (MOE) by about 1100 types of olfactory receptors (OR) expressed by olfactory sensory neurons (OSNs). Each mature OSN is thought to express only one allele of a single OR gene. Major impediments to understand the transcriptional control of OR gene expression are the lack of a proper characterization of OR transcription start sites (TSSs) and promoters, and of regulatory transcripts at OR loci. We have applied the nanoCAGE technology to profile the transcriptome and the active promoters in the MOE. nanoCAGE analysis revealed the map and architecture of promoters for 87.5% of the mouse OR genes, as well as the expression of many novel noncoding RNAs including antisense transcripts. We identified candidate transcription factors for OR gene expression and among them confirmed by chromatin immunoprecipitation the binding of TBP, EBF1 (OLF1), and MEF2A to OR promoters. Finally, we showed that a short genomic fragment flanking the major TSS of the OR geneOlfr160(M72) can drive OSN-specific expression in transgenic mice.
- Max Planck Society Germany
- University of Queensland Australia
- Cornell University United States
- Vita-Salute San Raffaele University Italy
- University College London United Kingdom
Biochemistry & Molecular Biology, 570, 3' Untranslated Regions; Animals; Base Sequence; Binding Sites; Consensus Sequence; DNA-Binding Proteins/metabolism; Gene Expression Profiling; Gene Expression Regulation; Gene Order; Genetic Loci; MEF2 Transcription Factors; Mice; Mice, Transgenic; Myogenic Regulatory Factors/metabolism; Olfactory Mucosa/metabolism; Olfactory Receptor Neurons/metabolism; Promoter Regions, Genetic; Receptors, Odorant/genetics; TATA-Box Binding Protein/metabolism; Transcription Factors/metabolism; Transcription Initiation Site; Transcription, Genetic, /dk/atira/pure/subjectarea/asjc/1300/1311, 572, 610, Mice, Transgenic, Receptors, Odorant, Olfactory Receptor Neurons, Mice, Olfactory Mucosa, Consensus Sequence, Gene Order, Genetics, Animals, Genetics(clinical), Promoter Regions, Genetic, 3' Untranslated Regions, MOE, antisense RNA, Genetics & Heredity, transcriptome; MOE; promoters; antisense RNA; nanoCAGE; TSS, promoter, Binding Sites, Base Sequence, MEF2 Transcription Factors, Gene Expression Profiling, nanoCAGE, /dk/atira/pure/subjectarea/asjc/2700/2716, TATA-Box Binding Protein, TSS, DNA-Binding Proteins, Biotechnology & Applied Microbiology, Gene Expression Regulation, Myogenic Regulatory Factors, Cardiovascular and Metabolic Diseases, Genetic Loci, Technology Platforms, transcriptome, Transcription Factors
Biochemistry & Molecular Biology, 570, 3' Untranslated Regions; Animals; Base Sequence; Binding Sites; Consensus Sequence; DNA-Binding Proteins/metabolism; Gene Expression Profiling; Gene Expression Regulation; Gene Order; Genetic Loci; MEF2 Transcription Factors; Mice; Mice, Transgenic; Myogenic Regulatory Factors/metabolism; Olfactory Mucosa/metabolism; Olfactory Receptor Neurons/metabolism; Promoter Regions, Genetic; Receptors, Odorant/genetics; TATA-Box Binding Protein/metabolism; Transcription Factors/metabolism; Transcription Initiation Site; Transcription, Genetic, /dk/atira/pure/subjectarea/asjc/1300/1311, 572, 610, Mice, Transgenic, Receptors, Odorant, Olfactory Receptor Neurons, Mice, Olfactory Mucosa, Consensus Sequence, Gene Order, Genetics, Animals, Genetics(clinical), Promoter Regions, Genetic, 3' Untranslated Regions, MOE, antisense RNA, Genetics & Heredity, transcriptome; MOE; promoters; antisense RNA; nanoCAGE; TSS, promoter, Binding Sites, Base Sequence, MEF2 Transcription Factors, Gene Expression Profiling, nanoCAGE, /dk/atira/pure/subjectarea/asjc/2700/2716, TATA-Box Binding Protein, TSS, DNA-Binding Proteins, Biotechnology & Applied Microbiology, Gene Expression Regulation, Myogenic Regulatory Factors, Cardiovascular and Metabolic Diseases, Genetic Loci, Technology Platforms, transcriptome, Transcription Factors
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).56 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!