publication . Article . Other literature type . 2021

Gene dosage compensation of rRNA transcript levels in Arabidopsis thaliana lines with reduced ribosomal gene copy number

Francesca Lopez; Antoine Fort; Luca Tadini; Aline V. Probst; Marcus McHale; James Friel; Peter Ryder; Frédéric Pontvianne; Paolo Pesaresi; Ronan Sulpice; ...
Open Access
  • Published: 02 Feb 2021 Journal: The Plant Cell (eissn: 1532-298X, Copyright policy)
  • Publisher: Oxford University Press (OUP)
Abstract
Abstract The 45S rRNA genes (rDNA) are amongst the largest repetitive elements in eukaryotic genomes. rDNA consists of tandem arrays of rRNA genes, many of which are transcriptionally silenced. Silent rDNA repeats may act as ‘back-up’ copies for ribosome biogenesis and have nuclear organization roles. Through Cas9-mediated genome editing in the Arabidopsis thaliana female gametophyte we reduced 45S rDNA copy number to a plateau of ∼10%. Two independent lines had rDNA copy numbers reduced by up to 90% at the T7 generation, named Low Copy Number (LCN) lines. Despite drastic reduction of rDNA copies, rRNA transcriptional rates and steady-state levels remained the s...
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free text keywords: Plant Science, Cell Biology, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Settore BIO/18 - Genetica, Regular Issue, Gene dosage, Copy-number variation, Genetics, Segmental duplication, Biology, Low copy number, Genome, Dosage compensation, Ribosomal RNA, Gene
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