Copernicus scans of selected interstellar absorption lines in the UV spectrum of Gamma Vel are analyzed, together with ground-based data, to obtain column densities for various ion states of C, N, O, Na, Mg, Al, Si, P, S, Cl, Ar, Ca, Mn, Fe, and CO. N I and O I are fitted to a single empirical curve of growth with a velocity parameter (b) of 8 km/s; Mg II, Si II, P II, S II, Mn II, and Fe II are fitted to another curve with b between 3 and 9 km/s. Abundance determinations relative to H I show that: (1) C, N, P, S, and Ar are probably close to their solar values; (2) O may be depleted by about a factor of 2; (3) Mg, Al, Si, Cl, Mn, and Fe are depleted by a factor of 4 or more: (4) Al is depleted by at least a factor of 10 in the H II region; and (5) both N V and O VI are present, but not C IV. The N V/O VI ratio implies that the electron temperature in the H II region is about 275,000 K.