Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation
Copyright policy )Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation
Focal adhesions (FAs) are mechanosensitive adhesion and signaling complexes that grow and change composition in response to myosin II–mediated cytoskeletal tension in a process known as FA maturation. To understand tension-mediated FA maturation, we sought to identify proteins that are recruited to FAs in a myosin II–dependent manner and to examine the mechanism for their myosin II–sensitive FA association. We find that FA recruitment of both the cytoskeletal adapter protein vinculin and the tyrosine kinase FA kinase (FAK) are myosin II and extracellular matrix (ECM) stiffness dependent. Myosin II activity promotes FAK/Src-mediated phosphorylation of paxillin on tyrosines 31 and 118 and vinculin association with paxillin. We show that phosphomimic mutations of paxillin can specifically induce the recruitment of vinculin to adhesions independent of myosin II activity. These results reveal an important role for paxillin in adhesion mechanosensing via myosin II–mediated FAK phosphorylation of paxillin that promotes vinculin FA recruitment to reinforce the cytoskeletal ECM linkage and drive FA maturation.
- University of California, San Diego United States
- National Institutes of Health United States
- University of California System United States
- National Institute of Health Pakistan
- National Institute of Health (NIH/NICHD) United States
570, 571, Pyridines, biological activity, cell maturation, 612, Heterocyclic Compounds, 4 or More Rings, genetics development and cell biology, Institute for Research in Electronics and Applied Physics, Marine Biological Laboratory, Mice, Structure-Activity Relationship, myosin II, Animals, focal adhesion, Phosphorylation, Research Articles, Cells, Cultured, mechanotransduction, Moores Cancer Center, Myosin Type II, paxillin, Focal Adhesions, Genetics and Genomics, protein tyrosine kinase, cytoskeleton, Chemical Engineering, Fibroblasts, Protein-Tyrosine Kinases, Amides, Vinculin, Extracellular Matrix, Fibronectins, Reproductive Medicine, Biological Engineering, national heart lung and blood institute, cell biology and physiology center, mutation, Paxillin
570, 571, Pyridines, biological activity, cell maturation, 612, Heterocyclic Compounds, 4 or More Rings, genetics development and cell biology, Institute for Research in Electronics and Applied Physics, Marine Biological Laboratory, Mice, Structure-Activity Relationship, myosin II, Animals, focal adhesion, Phosphorylation, Research Articles, Cells, Cultured, mechanotransduction, Moores Cancer Center, Myosin Type II, paxillin, Focal Adhesions, Genetics and Genomics, protein tyrosine kinase, cytoskeleton, Chemical Engineering, Fibroblasts, Protein-Tyrosine Kinases, Amides, Vinculin, Extracellular Matrix, Fibronectins, Reproductive Medicine, Biological Engineering, national heart lung and blood institute, cell biology and physiology center, mutation, Paxillin
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