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Preparative Biochemistry & Biotechnology
Article . 2016 . Peer-reviewed
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Comparative study of stability of soluble and cell wall invertase fromSaccharomyces cerevisiae

Authors: Margetić, Aleksandra; Vujčić, Zoran;

Comparative study of stability of soluble and cell wall invertase fromSaccharomyces cerevisiae

Abstract

Yeast Saccharomyces cerevisiae is the most significant source of enzyme invertase. It is mainly used in the food industry as a soluble or immobilized enzyme. The greatest amount of invertase is located in the periplasmic space in yeast. In this work, it was isolated into two forms of enzyme from yeast S. cerevisiae cell, soluble and cell wall invertase (CWI). Both forms of enzyme showed same temperature optimum (60 degrees C), similar pH optimum, and kinetic parameters. The significant difference between these biocatalysts was observed in their thermal stability, stability in urea and methanol solution. At 60 degrees C, CWI had 1.7 times longer half-life than soluble enzyme, while at 70 degrees C CWI showed 8.7 times longer half-life than soluble enzyme. After 2-hr of incubation in 8M urea solution, soluble invertase and CWI retained 10 and 60% of its initial activity, respectively. During 22hr of incubation of both enzymes in 30 and 40% methanol, soluble invertase was completely inactivated, while CWI changed its activity within the experimental error. Therefore, soluble invertase and CWI have not shown any substantial difference, but CWI showed better thermal stability and stability in some of the typical protein-denaturing agents.

This is a peer-reviewed version of the article: [https://doi.org/10.1080/10826068.2016.1244683]

[http://cer.ihtm.bg.ac.rs/handle/123456789/2185]

Country
Serbia
Subjects by Vocabulary

Microsoft Academic Graph classification: Immobilized enzyme Saccharomyces cerevisiae Cell wall chemistry.chemical_compound chemistry.chemical_classification biology Periplasmic space biology.organism_classification Yeast Invertase Enzyme chemistry Biochemistry Urea

Keywords

Protein Denaturation, urea, Saccharomyces cerevisiae, Biochemistry, Cell Wall, Enzyme Stability, Urea, enzyme stability, methanol, beta-Fructofuranosidase, Methanol, Temperature, General Medicine, Kinetics, Solubility, Cell wall invertase, Biotechnology

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download
citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
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9
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