publication . Article . 2007

Characterization of Pancreatic Transcription Factor Pdx-1 Binding Sites Using Promoter Microarray and Serial Analysis of Chromatin Occupancy

David M. Keller; Shannon K. McWeeney; Athanasios Arsenlis; Jacques Drouin; Christopher V.E. Wright; Haiyan Wang; Claes B. Wollheim; Peter White; Klaus H. Kaestner; Richard H. Goodman;
Open Access
  • Published: 31 Aug 2007 Journal: Journal of Biological Chemistry, volume 282, pages 32,084-32,092 (issn: 0021-9258, Copyright policy)
  • Publisher: Elsevier BV
  • Country: Switzerland
Abstract
The homeobox transcription factor Pdx-1 is necessary for pancreas organogenesis and beta cell function, however, most Pdx-1-regulated genes are unknown. To further the understanding of Pdx-1 in beta cell biology, we have characterized its genomic targets in NIT-1 cells, a mouse insulinoma cell line. To identify novel targets, we developed a microarray that includes traditional promoters as well as non-coding conserved elements, micro-RNAs, and elements identified through an unbiased approach termed serial analysis of chromatin occupancy. In total, 583 new Pdx-1 target genes were identified, many of which contribute to energy sensing and insulin release in pancre...
Persistent Identifiers
Subjects
Medical Subject Headings: endocrine systemhormones, hormone substitutes, and hormone antagonists
free text keywords: Cell Biology, Biochemistry, Molecular Biology, Animals, Basic Helix-Loop-Helix Transcription Factors/metabolism, Binding Sites, Cell Differentiation, Chromatin/metabolism, Chromatin Immunoprecipitation, Homeodomain Proteins/metabolism, Insulin, Insulin-Secreting Cells/cytology/metabolism, Mice, Mice, Inbred C57BL, MicroRNAs/metabolism, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, Promoter Regions, Genetic, Trans-Activators/metabolism, Transcription, Genetic, Pancreas, medicine.anatomical_structure, medicine, Promoter, Homeobox, Cell biology, Molecular biology, NEUROD1, Gene, Biology, Chromatin, Beta cell, Transcription factor, ddc:612
Related Organizations
Funded by
NIH| Novel Reagents For Beta Cell Biology
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 5U01DK072477-02
  • Funding stream: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
,
SNSF| Molecular Mechanisms of Beta-Cell Signal Transduction in Insulin Secretion and Interactions with Islet Alpha-Cells.
Project
  • Funder: Swiss National Science Foundation (SNSF)
  • Project Code: 3200-066907
  • Funding stream: Project funding | Project funding (Div. I-III)
,
NIH| FUNCTIONAL GENOMICS OF THE BETA-CELL
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 5U01DK056947-08
  • Funding stream: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
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