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Biomicrofluidics
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Europe PubMed Central
Other literature type . 2014
Data sources: PubMed Central
Biomicrofluidics
Article . 2014 . Peer-reviewed
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Fraunhofer-ePrints
Article . 2014
Data sources: Fraunhofer-ePrints
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A high-throughput cellulase screening system based on droplet microfluidics

Authors: Raluca Ostafe; Radivoje Prodanovic; W. Lloyd Ung; David A. Weitz; Rainer Fischer;

A high-throughput cellulase screening system based on droplet microfluidics

Abstract

A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells. (C) 2014 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.

Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3666]

Countries
Serbia, Germany
Subjects by Vocabulary

Microsoft Academic Graph classification: Population Microfluidics Cellulase law.invention law education Throughput (business) education.field_of_study Chromatography biology Chemistry Molecular biophysics Sorting Lab-on-a-chip Fluorescence Biochemistry biology.protein

Keywords

Biomedical Engineering, Colloid and Surface Chemistry, General Materials Science, Fluid Flow and Transfer Processes, Condensed Matter Physics, Fast Track

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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