Preparation of [32P]phosphatidylcholine and [32P]lysophosphatidylcholine by using soya beans

descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 1979 English Publisher:Portland Press Ltd.Journal:Biochemical Journal, volume 179, pages 713-714 (issn: 0264-6021,

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Authors: F H, Hubmann;

doi: 10.1042/bj1790713

pmid: 573119

pmc: PMC1186683

Preparation of [32P]phosphatidylcholine and [32P]lysophosphatidylcholine by using soya beans

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Abstract

This method describes a procedure that can be carried out easily to obtain large amounts of [32P]phosphatidylcholine and [32P]lysophosphatidylcholine. The method involves germinating soya beans in the presence of [32P]Pi. The yield was 0.58% for [P]phosphatidylcholine and 0.52% for [32P]lysophosphatidylcholine, and the specific radioactivity of both was 10(7) d.p.m./mumol.

Related Organizations

Max Planck Society
Germany
Max Planck Institute of Biochemistry
Germany

Keywords

Glycine max, Hydrolysis, Isotope Labeling, Phosphatidylcholines, Lysophosphatidylcholines, Phosphorus Radioisotopes

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