descriptionPublicationkeyboard_double_arrow_right Article 01 Nov 1974 English Publisher:Portland Press Ltd.Journal:Biochemical Journal, volume 143, pages 491-493 (issn: 0264-6021,

Authors: Barbara M. McIlroy; George T. Stevenson;

doi: 10.1042/bj1430491

pmid: 4218955

pmc: PMC1168411

Proteolysis of rabbit immunoglobulin M by papain

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Abstract

1. Digestion of rabbit immunoglobulin M (IgM) by papain for 5h, in the absence of reducing agent, gives Fabμ and Fc5μ fragments in high yield. Shorter periods give fragment Fc5μ with one or more Fabμ fragments still attached. 2. Reducing agent in the absence of a denaturant cleaves rabbit IgM into half-subunits, each containing one μ chain and one light chain. Digestion by papain in the presence of such a reducing agent destroys the Fcμ domains.

Keywords

Immunodiffusion, Hydrolysis, Proteins, Immunoglobulin Fc Fragments, Immunoglobulin Fab Fragments, Immunoglobulin M, Papain, Chromatography, Gel, Animals, Rabbits, Oxidation-Reduction

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