Azirine ligation: fast and selective protein conjugation via photoinduced azirine–alkene cycloaddition

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2010 English Publisher:Royal Society of Chemistry (RSC)Journal:Chemical Communications, volume 46, page 7,993 (issn: 1359-7345, eissn: 1364-548X,

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Authors: Reyna K V, Lim; Qing, Lin;

doi: 10.1039/c0cc02863k

pmid: 20865197

pmc: PMC2964347

Azirine ligation: fast and selective protein conjugation via photoinduced azirine–alkene cycloaddition

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Abstract

We report a new bioorthogonal ligation reaction between p-nitrodiphenylazirine and dimethyl fumarate. This photoinduced azirine-alkene cycloaddition provides a rapid (~2 min) and highly selective route to protein conjugation at neutral pH and room temperature in biological medium.

Related Organizations

University at Buffalo, State University of New York
United States
State University of New York at Potsdam
United States

Keywords

Spectrometry, Mass, Electrospray Ionization, Azirines, Cyclization, Photochemistry, Proteins, Alkenes

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