
AbstractWe demonstrate a simple and efficient method for producing ultrathin Bessel (‘non-diffracting’) light sheets of any color using a line-shaped beam and an annulus filter. With this robust and cost-effective technology, we obtained two-color, 3D images of biological samples with lateral/axial resolution of 250 nm/400 nm and high-speed, 4D volume imaging of 20 μm sized live sample at 1 Hz temporal resolution.
Light-sheet microscopy, Fluorescence imaging, Article
Light-sheet microscopy, Fluorescence imaging, Article
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