Light-sheet fluorescence microscopy for quantitative biology

descriptionPublicationkeyboard_double_arrow_right Article 30 Dec 2014 English Publisher:Springer Science and Business Media LLCJournal:Nature Methods, volume 12, pages 23-26 (issn: 1548-7091, eissn: 1548-7105,

Copyright policy )Funded by:DFG | Macromolecular Complexes ...

Authors: Ernst H K Stelzer;

doi: 10.1038/nmeth.3219

pmid: 25549266

Light-sheet fluorescence microscopy for quantitative biology

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Abstract

In light sheet–based fluorescence microscopy (LSFM), optical sectioning in the excitation process minimizes fluorophore bleaching and phototoxic effects. Because biological specimens survive long-term three-dimensional imaging at high spatiotemporal resolution, LSFM has become the tool of choice in developmental biology.

Related Organizations

Goethe University Frankfurt
Germany

Keywords

Tribolium, Microscopy, Confocal, Light, Lasers, Imaging, Three-Dimensional, Microscopy, Fluorescence, Animals, Developmental Biology, Fluorescent Dyes

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