Isolation of Pure lac Operon DNA*

descriptionPublicationkeyboard_double_arrow_right Article 01 Nov 1969 English Publisher:Springer Science and Business Media LLCJournal:Nature, volume 224, pages 768-774 (issn: 0028-0836, eissn: 1476-4687,

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Authors: J, Shapiro; L, Machattie; L, Eron; G, Ihler; K, Ippen; J, Beckwith;

doi: 10.1038/224768a0

pmid: 4902927

Isolation of Pure lac Operon DNA*

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Abstract

Two specialized transducing phages, which carry the lac operon of Escherichia coli inserted into their DNA in opposite orientations, are used as a source of complementary sequences from which to prepare pure lac operon duplex. The availability of a single pure promoter will facilitate many new experiments on genetic transcription.

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Keywords

DNA, Bacterial, DNA, Chromosomes, Bacterial, Coliphages, Microscopy, Electron, Genetic Code, Transduction, Genetic, DNA, Viral, Operon, Escherichia coli, Methods, Hybridization, Genetic, RNA, Messenger

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