publication . Article . 2011

Gray wolf (Canis lupus) is a natural definitive host for Neospora caninum.

Jitender P. Dubey; Mark C. Jenkins; C. Rajendran; K. B. Miska; L.R. Ferreira; Jorge A. Martins; Oliver C.H. Kwok; S. Choudhary;
Open Access
  • Published: 23 May 2011
  • Publisher: Veterinary parasitology
Abstract
None: The gray wolf (Canis lupus) was found to be a new natural definitive host for Neospora caninum. Neospora-like oocysts were found microscopically in the feces of three of 73 wolves from Minnesota examined at necropsy. N. caninum-specific DNA was amplified from the oocysts of all three wolves. Oocysts from one wolf were infective for the gamma interferon gene knock out (KO) mice. Viable N. caninum (designated NcWolfUS1) was isolated in cell cultures seeded with tissue homogenate from the infected mouse. Typical thick walled tissue cysts were found in outbred mice inoculated with the parasite from the KO mouse. Tissue stages in mice stained positively with N....
Persistent Identifiers
Subjects
Medical Subject Headings: parasitic diseasesfungi
free text keywords: General Veterinary, General Medicine, Parasitology, Canis, biology.organism_classification, biology, Polymerase chain reaction, law.invention, law, Feces, Parasite hosting, Virology, Gray wolf, ved/biology.organism_classification_rank.species, ved/biology, Polyclonal antibodies, biology.protein, Neospora caninum, Sylvatic cycle
Related Organizations
Abstract
None: The gray wolf (Canis lupus) was found to be a new natural definitive host for Neospora caninum. Neospora-like oocysts were found microscopically in the feces of three of 73 wolves from Minnesota examined at necropsy. N. caninum-specific DNA was amplified from the oocysts of all three wolves. Oocysts from one wolf were infective for the gamma interferon gene knock out (KO) mice. Viable N. caninum (designated NcWolfUS1) was isolated in cell cultures seeded with tissue homogenate from the infected mouse. Typical thick walled tissue cysts were found in outbred mice inoculated with the parasite from the KO mouse. Tissue stages in mice stained positively with N....
Persistent Identifiers
Subjects
Medical Subject Headings: parasitic diseasesfungi
free text keywords: General Veterinary, General Medicine, Parasitology, Canis, biology.organism_classification, biology, Polymerase chain reaction, law.invention, law, Feces, Parasite hosting, Virology, Gray wolf, ved/biology.organism_classification_rank.species, ved/biology, Polyclonal antibodies, biology.protein, Neospora caninum, Sylvatic cycle
Related Organizations
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