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Nano Communication Networks; ZENODO
Other literature type . Article . Journal . 2018 . Peer-reviewed
License: Elsevier TDM
ResearchGate Data
Preprint . 2018
Data sources: Datacite
https://doi.org/10.48550/arxiv...
Article . 2018
License: arXiv Non-Exclusive Distribution
Data sources: Datacite
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unimolecular fret sensors simple linker designs and properties

Authors: Sanyal, Shourjya; Coker, David F.; MacKernan, Donal;

unimolecular fret sensors simple linker designs and properties

Abstract

Protein activation and deactivation is central to a variety of biological mechanisms, including cellular signaling and transport. Unimolecular fluorescent resonance energy transfer (FRET) probes are a class of fusion protein sensors that allow biologists to visualize using an optical microscope whether specific proteins are activated due to the presence nearby of small drug-like signaling molecules, ligands or analytes. Often such probes comprise a donor fluorescent protein attached to a ligand binding domain, a sensor or reporter domain attached to the acceptor fluorescent protein, with these ligand binding and sensor domains connected by a protein linker. Various choices of linker type are possible ranging from highly flexible proteins to hinge-like proteins. It is also possible to select donor and acceptor pairs according to their corresponding F��ster radius, or even to mutate binding and sensor domains so as to change their binding energy in the activated or inactivated states. The focus of the present work is the exploration through simulation of the impact of such choices on sensor performance.

Keywords

Quantitative Biology - Biomolecules, Biological Physics (physics.bio-ph), FOS: Biological sciences, FOS: Physical sciences, Biomolecules (q-bio.BM), Physics - Biological Physics

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green
bronze