publication . Article . 2019

Preservation of female genetic resources of common carp through oogonial stem cell manipulation

Franěk, Roman; Tichopád, Tomáš; Steinbach, Christoph; Xie, Xuan; Lujić, Jelena; Marinović, Zoran; Horváth, Ákos; Kašpar, Vojtěch; Pšenička, Martin;
Open Access
  • Published: 01 Apr 2019
Abstract
Abstract Several experiments were conducted in order to develop an optimal protocol for slow-rate freezing (−1 °C/min) and short-term storage (−80 or 4 °C) of common carp ovarian tissue fragments with an emphasis on oogonial stem cells (OSCs). Dimethyl sulfoxide (Me2SO) with concentration of 1.5 M was identified as the best cryoprotectant in comparison to propylene glycol and methanol. When comparing supplementation of sugars (glucose, trehalose, sucrose) in different concentrations (0.1, 0.3, 0.5 M), glucose and trehalose in 0.3 M were identified as optimal. Short-term storage options for ovarian tissue pieces at −80 °C and 4 °C were tested as alternatives to c...
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Subjects
free text keywords: General Agricultural and Biological Sciences, General Biochemistry, Genetics and Molecular Biology, General Medicine, Cryopreservation, Oogonial Stem Cells, Cryoprotectant, Transplantation, Andrology, Trehalose, chemistry.chemical_compound, chemistry, Common carp, Carp, biology.organism_classification, biology, Dimethyl sulfoxide
Funded by
EC| AQUAEXCEL2020
Project
AQUAEXCEL2020
AQUAculture infrastructures for EXCELlence in European fish research towards 2020
  • Funder: European Commission (EC)
  • Project Code: 652831
  • Funding stream: H2020 | RIA
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