publication . Article . 1992

Pregnenolone sulfate augments NMDA receptor mediated increases in intracellular Ca2+ in cultured rat hippocampal neurons

Robert P. Irwin; Nicholas J. Maragakis; Michael A. Rogawski; Robert H. Purdy; David H. Farb; Steven M. Paul;
Open Access
  • Published: 01 Jul 1992 Journal: Neuroscience Letters, volume 141, pages 30-34 (issn: 0304-3940, Copyright policy)
  • Publisher: Elsevier BV
Abstract
The ability of the neuroactive steroid pregnenolone sulfate to alter N-methyl-D-aspartate (NMDA) receptor-mediated elevations in intracellular Ca2+ ([Ca2+]i) was studied in cultured fetal rat hippocampal neurons using microspectrofluorimetry and the Ca2+ sensitive indicator fura-2. Pregnenolone sulfate (5-250 microM) caused a concentration-dependent and reversible potentiation of the rise (up to approximately 800%) in [Ca2+]i induced by NMDA. In contrast, the steroid failed to alter basal (unstimulated) [Ca2+]i or to modify the rise in [Ca2+]i that occurs when hippocampal neurons are depolarized by high K+ in the presence of the NMDA receptor antagonist CPP. The...
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Medical Subject Headings: nervous system
free text keywords: General Neuroscience, Pregnenolone sulfate, chemistry.chemical_compound, chemistry, Neuroactive steroid, Pregnenolone, medicine.drug, medicine, Long-term potentiation, Endocrinology, medicine.medical_specialty, Epipregnanolone, Glutamate receptor, Internal medicine, NMDA receptor, Glutamic acid
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