Gamete recognition and binding are mediated by specific proteins on the surface of the sperm and egg. Identification and characterization of some of these proteins from several model systems, particularly mouse and sea urchin, have focused interest on the general properties and functions of gamete recognition proteins. Sperm-binding proteins located in egg extracellular coats as well as sperm-binding proteins that are localized to the egg plasma membrane are presented in the context of their structure and function in gamete binding. Unifying and disparate characteristics are discussed in light of the diverse biology of fertilization among species. Outstanding questions, alternative mechanisms and models, and strategies for future work are presented.