Name: DNAase I footprinting of restriction enzymes
Creator: Keith R. Fox
Keywords: 0301 basic medicine, 0303 health sciences, 03 medical and health sciences, Deoxyribonuclease I, Calcium, DNA, DNA Restriction Enzymes, Buffers, Deoxyribonucleases, Type II Site-Specific, Deoxyribonuclease EcoRI

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1988 English Publisher:Elsevier BVJournal:Biochemical and Biophysical Research Communications, volume 155, pages 779-785 (issn: 0006-291X,

Authors: Keith R. Fox;

doi: 10.1016/s0006-291x(88)80563-1

pmid: 2844179

DNAase I footprinting of restriction enzymes

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Abstract

DNAase I footprinting of restriction enzymes has been achieved by using calcium containing digestion buffers so that the enzymes bind to but do not cleave DNA. EcoR1 produces a footprint 17 bases long, overestimating the region of contact with DNA by about 7-8 base pairs. Restriction enzymes HaeIII and HinP1 generate smaller footprints of 15 and 13 base pairs respectively.

Related Organizations

University of Southampton
United Kingdom

Keywords

Deoxyribonuclease I, Calcium, DNA, DNA Restriction Enzymes, Buffers, Deoxyribonucleases, Type II Site-Specific, Deoxyribonuclease EcoRI

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