Abstract Milk samples of goats were examined for the presence of Mycobacterium avium subspecies paratuberculosis (Map) by culture method. Map was cultivated from the milk sample of clinically infected goat with Johne’s disease (JD). Study indicates excretion of Map bacilli in the milk, which may act as a source of infection to young kids. Present studies are the first report of isolation of cultivable Map from milk of Johne’s disease-infected goats in India.