Variations in relief associated with pedogenetic processes promote different intensities in weathering of sediments of the Barreiras Formation and may thus lead to the formation of different soil types, like Podzols, Acrisols and Ferralsols. The Podzols of tropical regions contain important information on climate and vegetation changes that occurred mainly in late Pleistocene and Holocene; however few studied, regarding their spatial variation, that can be investigated through ground penetrating radar (GPR). The aim was to study morphological, physical, chemical, stable C isotopic properties and spatial distribution of soils within depressions of the Barreiras Formation and characterize the 14C chronology of two Podzols and their B spodic horizons, along a transect grassland to forest in northeastern Espírito Santo State, Brazil. The profiles encompass a sequence of A-E-Bhm horizons, except for P3 and P6 with histic H and A-Bt, respectively. The GPR images showed patterns corresponding to these soil horizons, and the GPR data reveal the presence of diagnostic subsurface horizons characteristic of spodic horizons with cemented layers. The influence of relief factors and original materials was observed, associated with ferrolysis and podzolisation as main actors in the genesis of soils studied. The monomorphic organic matter filling the voids evidences the processes of imobilization, illuviation and precipitation, with the genesis of the spodic horizon. The Podzols profiles of Pleistocene organic matter ages accumulated compounds of C3 plants from the vegetation cover in the B spodic horizons of the profiles P4 and P1, since at least 14,251 and 38,890 cal BP, respectively, suggesting the dominance of a humid climate at least during the studied period in the region.