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Journal of Hazardous Materials
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Journal of Hazardous Materials
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Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes

Authors: Barbara Janović; Andrew Collins; Zoran Vujčić; Miroslava Vujčić;

Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes

Abstract

The aim of this study was to investigate the impact of dyes on DNA before and after enzymatic decolorization by acidic horseradish peroxidase (HRP-A). The comet assay is easy and feasible method widely used to measure DNA damage and repair. The medium-throughput comet assay was employed for assessment of genotoxic effects of 8 dyes in BEAS-2B cells. We have incorporated a digestion with bacterial endonuclease (formamidopyrimidine DNA glycosylase, FPG) to detect oxidized bases in the case of single and double azo dyes, Orange II (OR2) and Amido Black 10B (AB), respectively. This allowed detection 8-oxo7,8-dihydroguanine, one of most abundant oxidized bases in nuclear DNA. In the case of AB there was no indication of DNA damage, either strand brakes or FPG-sensitive sites before and after decolorization. The OR2 induced DNA damage (in terms of percentage of DNA in comet tails). Also, the frequency of FPG-sensitive sites increased with OR2 concentration. After decolorization no DNA damaging effects was seen at all. The interaction studies of OR2 and AB, before and after decolorization, with calf thymus DNA has been investigated by absorption and fluorescence spectroscopy. The results provide support for the idea that in some cases enzymatic decolorization contributes to lower genotoxicity potential. (C) 2016 Elsevier B.V. All rights reserved.

Peer-reviewed manuscript: [http://cherry.chem.bg.ac.rs/handle/123456789/3189]

Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3190]

Country
Serbia
Related Organizations
Subjects by Vocabulary

Microsoft Academic Graph classification: DNA damage Nanotechnology medicine.disease_cause Horseradish peroxidase Endonuclease chemistry.chemical_compound medicine biology Chemistry Formamidopyrimidine DNA glycosylase Nuclear DNA Comet assay Biochemistry biology.protein Genotoxicity DNA

Keywords

Environmental Engineering, Health, Toxicology and Mutagenesis, Color, Cell Line, Animals, Humans, Environmental Chemistry, Coloring Agents, Comet assay, Waste Management and Disposal, Horseradish Peroxidase, Horseradish peroxidase a, Decolorization, DNA, Pollution, Dye degradation, DNA damage, Cattle, Spectrophotometry, Ultraviolet, DNA interactions, Azo Compounds, Mutagens

44 references, page 1 of 5

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N. Lončar, B. Janović, M. Vujčić, Z. Vujčić, Decolorization of textile dyes and effluents using potato (Solanum tuberosum) phenoloxidase, Int. Biodeterior. Biodegrad. 72 (2012) 42-45.

doi:10.1016/j.ibiod.2012.05.001.

F.W. Krainer, A. Glieder, An updated view on horseradish peroxidases: recombinant production and biotechnological applications, Appl. Microbiol. Biotechnol. 99 (2015) 1611- 1625. doi:10.1007/s00253-014-6346-7. [OpenAIRE]

Q. Husain, Peroxidase mediated decolorization and remediation of wastewater containing industrial dyes: A review, Rev. Environ. Sci. Biotechnol. 9 (2010) 117-140.

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A. Bhunia, S. Durani, P.P. Wangikar, Horseradish Peroxidase Catalyzed Important Dyes, Biotechnol. Bioeng. 5 (2001) 562-567.

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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impulse
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