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International Journal of Biological Macromolecules
Article . 2021 . Peer-reviewed
License: Elsevier TDM
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Immobilization of yeast cell walls with surface displayed laccase from Streptomyces cyaneus within dopamine-alginate beads for dye decolorization

Authors: Popović, Nikolina; Pržulj, Dunja; Mladenović, Maja; Prodanović, Olivera; Ece, Selin; Ilić Đurđić, Karla; Ostafe, Raluca; +2 Authors

Immobilization of yeast cell walls with surface displayed laccase from Streptomyces cyaneus within dopamine-alginate beads for dye decolorization

Abstract

High amounts of toxic textile dyes are released into the environment due to coloring and wastewaters treatment processes' inefficiency. To remove dyes from the environment and wastewaters, researchers focused on applying immobilized enzymes due to mild reaction conditions and enzyme nontoxicity. Laccases are oxidases with wide substrate specificity, capable of degradation of many different dye types. Laccase from Streptomyces cyaneus was expressed on the surface of Saccharomyces cerevisiae EBY100 cells. The specific activity of surface-displayed laccase was increased by toluene-induced lysis to 3.1 U/g of cell walls. For cell wall laccase immobilization within hydrogel beads, alginate was modified by dopamine using periodate oxidation and reductive amination and characterized by UV–Vis, FTIR, and NMR spectroscopy. Cell wall laccase was immobilized within alginate and dopamine-alginate beads additionally cross-linked by oxygen and laccase. The immobilized enzyme's specific activity was two times higher using dopamine-alginate compared to native alginate beads, and immobilization yield increased 16 times. Cell wall laccase immobilized within dopamine-alginate beads decolorized Amido Black 10B, Reactive Black 5, Evans Blue, and Remazol Brilliant Blue with 100% efficiency and after ten rounds of multiple-use retained decolorization efficiency of 90% with Evans Blue and 61% with Amido Black.

This is the peer-reviewed version of the article: Popović, N.; Pržulj, D.; Mladenović, M.; Prodanović, O.; Ece, S.; Ilić Đurđić, K.; Ostafe, R.; Fischer, R.; Prodanović, R. Immobilization of Yeast Cell Walls with Surface Displayed Laccase from Streptomyces Cyaneus within Dopamine-Alginate Beads for Dye Decolorization. International Journal of Biological Macromolecules 2021, 181, 1072–1080. [https://doi.org/10.1016/j.ijbiomac.2021.04.115].

Supplementary material: [https://cherry.chem.bg.ac.rs/handle/123456789/4405]

Country
Serbia
Subjects by Vocabulary

Microsoft Academic Graph classification: Lysis Immobilized enzyme Cell wall chemistry.chemical_compound Laccase Periodate Yeast Amido black 10B chemistry Amido Black Nuclear chemistry

Keywords

Alginates, Dopamine, Biochemistry, Cell Wall, Structural Biology, Coloring Agents, Molecular Biology, Laccase, General Medicine, Streptomyces, Yeast surface display

40 references, page 1 of 4

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[3] D.M. Mate, M. Alcalde, Laccase: a multi-purpose biocatalyst at the forefront of biotechnology, Microb Biotechnol 10(6) (2017) 1457-1467.

[4] Z.K. Bagewadi, S.I. Mulla, H.Z. Ninnekar, Purification and immobilization of laccase from Trichoderma harzianum strain HZN10 and its application in dye decolorization, Journal of Genetic Engineering and Biotechnology 15(1) (2017) 139-150. [OpenAIRE]

[5] N. Jaiswal, V.P. Pandey, U.N. Dwivedi, Immobilization of papaya laccase in chitosan led to improved multipronged stability and dye discoloration, International Journal of Biological Macromolecules 86 (2016) 288-295.

[6] M. Fernandez-Fernandez, M.A. Sanroman, D. Moldes, Recent developments and applications of immobilized laccase, Biotechnology Advances 31(8) (2013) 1808-1825.

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[8] Y. Chen, B. Stemple, M. Kumar, N. Wei, Cell Surface Display Fungal Laccase as a Renewable Biocatalyst for Degradation of Persistent Micropollutants Bisphenol A and Sulfamethoxazole, Environmental Science & Technology 50(16) 8799-8808.

[9] M.T. Daâssi Dalel, Nasri Moncef, Rodriguez-Couto Susana, Decolorization of the metal textile dye Lanaset Grey G by immobilized white-rot fungi, Journal of Environmental Management 129 (2013) 324-32. [OpenAIRE]

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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views
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