Hyaluronan inhibits BMP‐induced osteoblast differentiation
Copyright policy )Hyaluronan inhibits BMP‐induced osteoblast differentiation
Hyaluronan (HA), one of the major structural extracellular components in cartilage, regulates cellular responses via receptors such as CD44. However, the direct effects of HA on osteoblastic differentiation has not been studied in detail. Here, we investigated the effects of HA (molecular weight: 900–1200kDa) on osteoblastic differentiation that was induced by bone morphogenetic protein (BMP) in C2C12 cells (mouse myoblastic cells) and ST2 cells (mouse bone marrow cells). BMP‐induced osteoblastic differentiation and Smad1/Smad5/Smad8 phosphorylation were downregulated by HA. Use of the CD44‐blocking antibody restored HA‐induced inhibition of osteoblastic differentiation and Smad1/Smad5/Smad8 phosphorylation. Our results indicate that HA inhibits BMP‐induced osteoblastic differentiation through the CD44 receptor.
- Osaka University Japan
- Osaka Gakuin University Japan
Adult, Male, rho GTP-Binding Proteins, Restriction Mapping, Bone Morphogenetic Protein 2, Bone morphogenetic protein, Cell Line, Mice, Osteoarthritis, Animals, Humans, CD44, Hyaluronic Acid, Hyaluronan, Aged, Osteoblasts, Osteoblast, Cell Differentiation, Middle Aged, Alkaline Phosphatase, Hyaluronan Receptors, Female, rhoA GTP-Binding Protein
Adult, Male, rho GTP-Binding Proteins, Restriction Mapping, Bone Morphogenetic Protein 2, Bone morphogenetic protein, Cell Line, Mice, Osteoarthritis, Animals, Humans, CD44, Hyaluronic Acid, Hyaluronan, Aged, Osteoblasts, Osteoblast, Cell Differentiation, Middle Aged, Alkaline Phosphatase, Hyaluronan Receptors, Female, rhoA GTP-Binding Protein
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).27 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!