Adipocyte- (A) and heart- (H) type fatty acid binding proteins (FABP) contribute to efficient fat storage and utilization, respectively. To understand regional-differences in lipid metabolism between tissues, A- and H-FABP transcript and protein levels were studied in adipocytes isolated from subcutaneous adipose tissue or skeletal muscle in growing pigs (Sus scrofa). Interestingly, H-FABP was expressed in adipocytes isolated from both sites. We also showed that A-FABP and H-FABP were expressed at a lower level in intramuscular adipocytes than in subcutaneous adipocytes. A discrepancy was observed between age-related changes in A-FABP content in isolated adipocytes and cell diameter or lipid content variations in tissues during growth.