High-throughput screening assay for d-amino acid oxidase
Copyright policy )High-throughput screening assay for d-amino acid oxidase
A membrane-based high-throughput screening (HTS) assay for active D-amino acid oxidase (DAAO) in liquid samples as well as in intact Escherichia coli cells has been developed and optimized. The detection limit of the assay was less than 1 ng per sample. The method proposed can be used for quantitative DAAO determination in the range of 0.13 to 3.60 ng enzyme per probe. The protocol was successfully tested to screen a library of E. coli clones containing mutant DAAOs active toward target substrates.
- Lomonosov Moscow State University Russian Federation
Collodion, Etorphine, Reproducibility of Results, Membranes, Artificial, Drug Combinations, Mutagenesis, Mutation, Escherichia coli, Methotrimeprazine, Amino Acid Oxidoreductases, Amino Acids
Collodion, Etorphine, Reproducibility of Results, Membranes, Artificial, Drug Combinations, Mutagenesis, Mutation, Escherichia coli, Methotrimeprazine, Amino Acid Oxidoreductases, Amino Acids
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