publication . Article . 1992

Pregnenolone sulfate augments NMDA receptor mediated increases in intracellular Ca2+ in cultured rat hippocampal neurons

Robert P. Irwin; Nicholas J. Maragakis; Michael A. Rogawski; Robert H. Purdy; David H. Farb; Steven M. Paul;
Open Access
  • Published: 01 Jul 1992 Journal: Neuroscience Letters, volume 141, pages 30-34 (issn: 0304-3940, Copyright policy)
  • Publisher: Elsevier BV
The ability of the neuroactive steroid pregnenolone sulfate to alter N-methyl-D-aspartate (NMDA) receptor-mediated elevations in intracellular Ca2+ ([Ca2+]i) was studied in cultured fetal rat hippocampal neurons using microspectrofluorimetry and the Ca2+ sensitive indicator fura-2. Pregnenolone sulfate (5-250 microM) caused a concentration-dependent and reversible potentiation of the rise (up to approximately 800%) in [Ca2+]i induced by NMDA. In contrast, the steroid failed to alter basal (unstimulated) [Ca2+]i or to modify the rise in [Ca2+]i that occurs when hippocampal neurons are depolarized by high K+ in the presence of the NMDA receptor antagonist CPP. These data suggest that the previously reported excitatory properties of pregnenolone sulfate may be due, in part, to an augmentation of the action of glutamic acid at the NMDA receptor.
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Medical Subject Headings: nervous system
free text keywords: General Neuroscience, Glutamate receptor, Epipregnanolone, Pregnenolone sulfate, chemistry.chemical_compound, chemistry, NMDA receptor, Long-term potentiation, Neuroactive steroid, Internal medicine, medicine.medical_specialty, medicine, Endocrinology, Pregnenolone, medicine.drug, Glutamic acid
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Article . 1992
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