Reversible inhibition of EcoRI with elevated pressure
Copyright policy )Reversible inhibition of EcoRI with elevated pressure
The endonuclease activity of EcoRI is completely inhibited at 200 MPa, 37 degrees C using the plasmid pBR 322 as a substrate. When assayed at 133 MPa approximately half the activity at atmospheric pressure was observed; from these data the standard molar volume change is estimated to be -80 cm3mol-1. Upon return to atmospheric pressure the enzyme reacted in a standard manner with its restriction site in pBR 322. Pressurization did not decrease the specificity of the endonuclease activity of the enzyme for its canonical site. These results are discussed in terms of the role of ionic interactions in protein-DNA interactions.
- Bell Labs United States
Atmospheric Pressure, Hydrostatic Pressure, Pressure, DNA, Deoxyribonuclease EcoRI, Plasmids
Atmospheric Pressure, Hydrostatic Pressure, Pressure, DNA, Deoxyribonuclease EcoRI, Plasmids
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