Studies on poly(adenosine diphosphate-ribose)
Copyright policy )Studies on poly(adenosine diphosphate-ribose)
Abstract Hydroxylapatite column chromatography was used for purification of poly (ADP-Rib). With an increasing concentration gradient of phosphate buffer (pH 6.8) RNA, DNA, and poly (ADP-Rib) with a chain of 17–18 ADP-ribose units were eluted successively. RNase and DNase digestion of a crude extract containing RNA, DNA, and poly(ADP-Rib) before chromatography resulted in a better separation of poly (ADP-Rib). Poly (ADP-Rib) with a shorter chain length were eluted with more dilute phosphate buffer and a linear relationship was obtained between the chain length of the material eluted and the phosphate concentration of the elution buffer.
- University of Tokyo Japan
- National Cancer Research Institute United Kingdom
Cell Nucleus, Carbon Isotopes, Chromatography, Deoxyribonucleases, Adenine Nucleotides, Nucleoside Diphosphate Sugars, Venoms, Polynucleotides, Snakes, Alkaline Phosphatase, Phosphoric Monoester Hydrolases, Phosphates, Rats, Molecular Weight, Ribonucleases, Liver, Animals, Hydroxyapatites
Cell Nucleus, Carbon Isotopes, Chromatography, Deoxyribonucleases, Adenine Nucleotides, Nucleoside Diphosphate Sugars, Venoms, Polynucleotides, Snakes, Alkaline Phosphatase, Phosphoric Monoester Hydrolases, Phosphates, Rats, Molecular Weight, Ribonucleases, Liver, Animals, Hydroxyapatites
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