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Histochemistry and Cell Biology
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ZENODO; Histochemistry and Cell Biology
Article . 2017 . Peer-reviewed
License: Springer TDM
Data sources: ZENODO; Crossref
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Expression of epithelial sodium channel (ENaC) and CFTR in the human epidermis and epidermal appendages

Authors: Israel Hanukoglu; Vijay Raju Boggula; Hananya Vaknine; Sachin Sharma; Thomas R. Kleyman; Aaron Hanukoglu;

Expression of epithelial sodium channel (ENaC) and CFTR in the human epidermis and epidermal appendages

Abstract

A major function of the skin is the regulation of body temperature by sweat secretions. Sweat glands secrete water and salt, especially NaCl. Excreted water evaporates, cooling the skin surface, and Na+ ions are reabsorbed by the epithelial sodium channels (ENaC). Mutations in ENaC subunit genes lead to a severe multi-system (systemic) form of pseudohypoaldosteronism (PHA) type I, characterized by salt loss from aldosterone target organs, including sweat glands in the skin. In this study, we mapped the sites of localization of ENaC in the human skin by confocal microscopy using polyclonal antibodies generated against human αENaC. Our results reveal that ENaC is expressed strongly in all epidermal layers except stratum corneum, and also in the sebaceous glands, eccrine glands, arrector pili smooth muscle cells, and intra-dermal adipocytes. In smooth muscle cells and adipocytes, ENaC is co-localized with F-actin. No expression of ENaC was detected in the dermis. CFTR is strongly expressed in sebaceous glands. In epidermal appendages noted, except the eccrine sweat glands, ENaC is mainly located in the cytoplasm. In the eccrine glands and ducts, ENaC and CFTR are located on the apical side of the membrane. This localization of ENaC is compatible with ENaC's role in salt reabsorption. PHA patients may develop folliculitis, miliaria rubra, and atopic dermatitis-like skin lesions, due to sweat gland duct occlusion and inflammation of eccrine glands as a result of salt accumulation.

Subjects by Vocabulary

Microsoft Academic Graph classification: Epithelial sodium channel medicine.medical_specialty Human skin Biology Dermis Internal medicine Renin–angiotensin system medicine Secretion Reabsorption Pseudohypoaldosteronism medicine.disease Cell biology medicine.anatomical_structure Endocrinology Epidermis

Keywords

Adult, Male, Histology, Adolescent, Cystic Fibrosis Transmembrane Conductance Regulator, Fluorescent Antibody Technique, Humans, Child, Epithelial Sodium Channels, Molecular Biology, Aged, Aged, 80 and over, Cell Biology, Middle Aged, Medical Laboratory Technology, Female, Epidermis

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
45
Top 10%
Top 10%
Top 10%
hybrid