Mapping bi-regional scientific collaboration demands multiple approaches to obtain a picture as complete as possible. Usually, the first approach is the measuring of the number and typology of scientific co-publications in the most visible indexes of journals and publications covered by databases like Web of Science or Scopus, among others. This paper analyzes scientific publications listed by Web of Science (WoS), which comprises authors from the 28 EU countries and Latin American and Caribbean countries (EULAC) between 2005 and 2016. The following questions have been addressed: How are bi-regional scientific relations between EULAC countries reflected by international collaboration? What effects does this scientific collaboration have in smaller or emerging countries? Which area of knowledge has more international collaborations? The study highlights the existence of a growing global network of researchers from several countries that collaborate on their research. EULAC scientific collaboration cannot be understood in isolation from this global network.