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Bioprocess and Biosystems Engineering
Article . 2013 . Peer-reviewed
License: Springer TDM
Data sources: Crossref
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Soybean hull peroxidase immobilization on macroporous glycidyl methacrylates with different surface characteristics

Authors: Prokopijević, Miloš; Prodanović, Olivera; Spasojević, Dragica; Stojanović, Željko; Radotić, Ksenija; Prodanović, Radivoje;

Soybean hull peroxidase immobilization on macroporous glycidyl methacrylates with different surface characteristics

Abstract

Soybean hull peroxidase (SHP, E.C. 1.11.1.7) was immobilized by a glutaraldehyde and periodate method onto series of macroporous copolymers of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA), poly(GMA-co-EGDMA) with various surface characteristics and pore size diameters ranging from 44 to 200 nm. Glutaraldehyde immobilization method and poly(GMA-co-EGDMA) named SGE 20/12 with pore sizes of 120 nm gave immobilized enzyme with highest specific activity of 25 U/g. Deactivation studies showed that immobilization increased stability of SHP and that surface characteristics of the used copolymer had a major influence on a stability of immobilized enzyme at high temperatures and in an organic solvent. The highest thermostability was obtained using the copolymer SGE 20/12 with pore size of 120 nm, while the highest stability in dioxane had SHP immobilized onto copolymer SGE 10/4 with pore size of 44 nm. Immobilized SHP showed a wider pH optimum as compared to the native enzyme especially at alkaline pH values and 3.2 times increased K (m) value for pyrogallol. After 6 cycles of repeated use in batch reactor, immobilized SHP retained 25 % of its original activity. Macroporous copolymers with different surface characteristics can be used for fine tuning of activity and stability of immobilized SHP to obtain a biocatalyst suitable for phenol oxidation or polymer synthesis in organic solvents.

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Serbia
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Subjects by Vocabulary

Microsoft Academic Graph classification: Glycidyl methacrylate Immobilized enzyme Ethylene glycol dimethacrylate chemistry.chemical_compound Polymer chemistry Copolymer Phenol Thermostability Periodate chemistry Glutaraldehyde Nuclear chemistry

Keywords

Hot Temperature, Periodate, Glycine max, Surface Properties, Bioengineering, Glutaraldehyde, Copolymer, Enzyme Stability, Peroxidase, Plant Proteins, General Medicine, Enzymes, Immobilized, Epoxy Compounds, Methacrylates, Immobilized enzyme, Stability, Biotechnology

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    Top 10%
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download
citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
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16
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