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Somatic embryogenesis in Encephalartos cycadifolius

descriptionPublicationkeyboard_double_arrow_right Article 01 Feb 1996Publisher:Springer Science and Business Media LLCJournal:Plant Cell Reports, volume 15, pages 437-440 (issn: 0721-7714, eissn: 1432-203X,

Authors: Anna K. Jäger; Johannes van Staden;

doi: 10.1007/s002990050049 , 10.1007/bf00232071

pmid: 24178425

Somatic embryogenesis in Encephalartos cycadifolius

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Abstract

Callus cultures of Encephalartos cycadifolius were established from zygotic embryo explants on a modified B5 medium containing 1 mg l(-1) 2,4-D and 1 mg l(-1) kinetin. Callus was transferred to media containing various combinations of 2,4-D and kinetin for improvement of somatic embryogenesis. Somatic embryos were produced on media with several growth regulator combinations. The somatic embryos developed from proembryos, which developed long suspensors. A dicotyledonary embryo formed at the distal end of the suspensor. The embryos turned green in light. When transferred to a medium containing 1 mg l(-1) ABA the somatic embryos matured. The suspensors desiccated and these embryos rooted when transferred to a medium without phytohormones.

Related Organizations

IE University
Spain
University of Natal
South Africa

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