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Applied Microbiology and Biotechnology
Article . 2016 . Peer-reviewed
License: Springer TDM
Data sources: Crossref
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Tyramine-modified pectins via periodate oxidation for soybean hull peroxidase induced hydrogel formation and immobilization

Authors: Miloš Prokopijević; Olivera Prodanović; Dragica Spasojević; Gordana Kovacevic; Natalija Polovic; Ksenija Radotić; Radivoje Prodanovic;

Tyramine-modified pectins via periodate oxidation for soybean hull peroxidase induced hydrogel formation and immobilization

Abstract

Pectin was modified by oxidation with sodium periodate at molar ratios of 2.5, 5, 10, 15 and 20 mol% and reductive amination with tyramine and sodium cyanoborohydride afterwards. Concentration of tyramine groups within modified pectin ranged from 54.5 to 538 mu mol/g of dry pectin while concentration of ionizable groups ranged from 3.0 to 4.0 mmol/g of dry polymer compared to 1.5 mmol/g before modification due to the introduction of amino group. All tyramine-pectins showed exceptional gelling properties and could form hydrogel both by cross-linking of carboxyl groups with calcium or by cross-linking phenol groups with peroxidase in the presence of hydrogen peroxide. These hydrogels were tested as carriers for soybean hull peroxidase (SHP) immobilization within microbeads formed in an emulsion based enzymatic polymerization reaction. SHP immobilized within tyramine-pectin microbeads had an increased thermal and organic solvent stability compared to the soluble enzyme. Immobilized SHP was more active in acidic pH region and had slightly decreased K (m) value of 2.61 mM compared to the soluble enzyme. After 7 cycles of repeated use in batch reactor for pyrogallol oxidation microbeads, immobilized SHP retained half of the initial activity.

Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3053]

Country
Serbia
Related Organizations
Subjects by Vocabulary

Microsoft Academic Graph classification: food.ingredient Immobilized enzyme Pectin Reductive amination chemistry.chemical_compound food Organic chemistry Hydrogen peroxide biology Sodium periodate Sodium cyanoborohydride Periodate chemistry biology.protein Nuclear chemistry Peroxidase

Keywords

Glycine max, Tyramine, Borohydrides, Applied Microbiology and Biotechnology, Equipment Reuse, Enzyme immobilization, Pectin modification, Peroxidase, Plant Proteins, Waste Products, Periodic Acid, Hydrogels, Hydrogen Peroxide, General Medicine, Hydrogen-Ion Concentration, Enzymes, Immobilized, Kinetics, Pectins, Soybean hull peroxidase, Oxidation-Reduction, Biotechnology

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    12
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    Top 10%
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visibility
citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
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12
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