The notion of an inverse transversal of a regular semigroup is well-known. Here we investigate naturally ordered regular semigroups that have an inverse transversal. Such semigroups are necessarily locally inverse and the inverse transversal is a quasi-ideal. After considering various general properties that relate the imposed order to the natural order, we highlight the situation in which the inverse transversal is a monoid. The regularity of Green’s relations is also characterised. Finally, we determine the structure of a naturally ordered regular semigroup with an inverse monoid transversal.