Epicuticular wax from sorghum seedling leaves was isolated and analyzed by TLC and GC. Its composition was remarkably similar to that of waxes on many other Gramineae leaves with the exception that three alkan-2-ol esters (nonan-2-ol docosanoate, nonan-2-ol tetracosanoate, and nonan-2-ol hexacosanoate) were present. This was unexpected as such esters have hitherto only been reported in waxes of plants with the capability of synthesizing β-diketone lipids. The C31−C35 alkan-2-ol esters were separated from the C38−C56 alkan-1-ol esters by preparative GC. The column effluent was passed through a small Dexsil-300 trap maintained at 150°C, and the esters eluted therefrom with chloroform. This technique gave better than 90% recovery of a [1-14C]−C32 ester.