PHB recovery by hypochlorite digestion of non-PHB biomass

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 1989 English Publisher:Springer Science and Business Media LLCJournal:Biotechnology Techniques, volume 3, pages 227-232 (issn: 0951-208X, eissn: 1573-6784,

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Authors: E. Berger; B. A. Ramsay; J. A. Ramsay; C. Chavarie; G. Braunegg;

doi: 10.1007/bf01876053

PHB recovery by hypochlorite digestion of non-PHB biomass

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Abstract

Hypochlorite digestion of bacterial biomass from intracellular poly-β-hydroxybutyrate (PHB) has not been used on a large scale since it has been reported to severely degrade PHB. In this study, to minimize degradation, the initial biomass concentration, digestion time and pH of the hypochlorite solution were optimized. Consequently, PHB of 95% purity with a weight average molecular weight (MW) of 600,000 and a polydispersity index (PI) of 4.5 was recovered from biomass initially containing PHB with a MW of 1,200,000 and a PI of 3.

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