descriptionPublicationkeyboard_double_arrow_right Article 01 Dec 1993 English Publisher:Springer Science and Business Media LLCJournal:Molecular and General Genetics MGG, volume 241-241, pages 719-722 (issn: 0026-8925, eissn: 1432-1874,

Authors: H. K. Das; Adhar C. Manna;

doi: 10.1007/bf00279916

pmid: 8264545

Determination of the size of the Azotobacter vinelandii chromosome

- Summary
- Subjects
- Metrics

Abstract

The chromosome of Azotobacter vinelandii UW was digested separately with the rape cutter restriction endonucleases SwaI (5'-ATTTAAAT), PmeI (5'GTTTAAAC) and PacI (5'-TTAATTAA) and the products were separated by pulsed-field gel electrophoresis. The size of the chromosome was determined to be approximately 4.5 megabase pairs (Mb) based on the sum of the sizes of the restriction fragments. This is almost the same as the size of the chromosome of Escherichia coli. The inability of the undigested DNA to enter the gel has led us to infer that the chromosome is circular.

Related Organizations

Jawaharlal Nehru University
India

Keywords

DNA, Bacterial, Azotobacter vinelandii, Restriction Mapping, Chromosomes, Bacterial, DNA, Circular, Electrophoresis, Gel, Pulsed-Field

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	14
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%