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Assays of the Helicase, ATPase, and Exoribonuclease Activities of the Yeast Mitochondrial Degradosome

descriptionPublicationkeyboard_double_arrow_right Part of book or chapter of book , Article 01 Jan 2009Publisher:Humana Press

Authors: Michal Malecki; Pawel Golik; Piotr P. Stepien;

doi: 10.1007/978-1-60327-355-8_24

pmid: 20225161

Assays of the Helicase, ATPase, and Exoribonuclease Activities of the Yeast Mitochondrial Degradosome

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Abstract

The mitochondrial degradosome (mtEXO) is the main enzymatic complex in RNA degradation, processing, and surveillance in Saccharomyces cerevisiae mitochondria. It consists of two nuclear-encoded subunits: the ATP-dependent RNA helicase Suv3p and the 3' to 5' exoribonuclease Dss1p. The two subunits depend on each other for their activity; the complex can therefore be considered as a model system for the cooperation of RNA helicases and exoribonucleases in RNA degradation. All the three activities of the complex (helicase, ATPase, and exoribonuclease) can be studied in vitro using recombinant proteins and protocols presented in this chapter.

Related Organizations

University of Warsaw
Poland

Keywords

Adenosine Triphosphatases, Polyribonucleotide Nucleotidyltransferase, Saccharomyces cerevisiae Proteins, Saccharomyces cerevisiae, Recombinant Proteins, Mitochondria, Protein Subunits, Multienzyme Complexes, Endoribonucleases, Exoribonucleases, Biological Assay, RNA Helicases

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6 Research products, page 1 of 1

Structural Studies of the Yeast Mitochondrial Degradosome
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In vitro Reconstitution and Characterization of the Yeast Mitochondrial Degradosome Complex Unravels Tight Functional Interdependence
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Exoribonuclease II, mitochondrial
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Chapter 22 In Vivo and In Vitro Approaches for Studying the Yeast Mitochondrial RNA Degradosome Complex
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ATP-dependent RNA helicase SUV3, mitochondrial
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The 3′ Ends of Mature Transcripts Are Generated by a Processosome Complex in Fission Yeast Mitochondria
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