Identification of Bifidobacteria by the Phosphoketolase Assay
Identification of Bifidobacteria by the Phosphoketolase Assay
Bifidobacteria are commensal bacteria, which naturally colonize the gastrointestinal tract of a large number of animals, including humans, contributing to their health and well-being. An important taxonomic marker for the identification of members of the bifidobacterial group is the presence of the fructose-6-phosphate phosphoketolase (F6PPK) activity. The F6PPK enzyme is involved in the bifidus shunt based on the ability of F6PPK to split fructose-6-phosphate into erythrose-4-phosphate and acetyl phosphate. Here, we describe the two main methods utilized to detect the presence of F6PPK activity, that is, the enzymatic assay and the presence of the D-xylulose-5-phosphate/fructose-6-phosphate phosphoketolase bifidobacterial gene.
Bacterial taxonomy; Bifidobacterium; Bifidus shunt; Fructose 6 phosphoketolase; Polymerase chain reaction, Bacterial taxonomy; Bifidobacterium; Bifidus shunt; Fructose 6 phosphoketolase; Polymerase chain reaction; Aldehyde-Lyases; Bacterial Proteins; Bacterial Typing Techniques; Bifidobacterium; Cell Culture Techniques; Enzyme Assays; Genes, Bacterial; Polymerase Chain Reaction, Cell Culture Techniques, Polymerase Chain Reaction, Bacterial Typing Techniques, Bacterial Proteins, Genes, Bacterial, Bifidobacterium, Aldehyde-Lyases, Enzyme Assays
Bacterial taxonomy; Bifidobacterium; Bifidus shunt; Fructose 6 phosphoketolase; Polymerase chain reaction, Bacterial taxonomy; Bifidobacterium; Bifidus shunt; Fructose 6 phosphoketolase; Polymerase chain reaction; Aldehyde-Lyases; Bacterial Proteins; Bacterial Typing Techniques; Bifidobacterium; Cell Culture Techniques; Enzyme Assays; Genes, Bacterial; Polymerase Chain Reaction, Cell Culture Techniques, Polymerase Chain Reaction, Bacterial Typing Techniques, Bacterial Proteins, Genes, Bacterial, Bifidobacterium, Aldehyde-Lyases, Enzyme Assays
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