Identification of Dh/ + and Dh/Dh embryos through close linkage of Dh and peptidase‐3
Copyright policy )Identification of Dh/ + and Dh/Dh embryos through close linkage of Dh and peptidase‐3
AbstractThe close linkage between the genes Dominant hemimelia (Dh) and peptidase‐3 (Pep‐3) has been determined in 65 informative matings with the recombination freqency of 3.8%. Progeny testing showed that non‐penetrance does occur in Dh/+ adults. The presence of the “slow” and “fast” variants of Pep‐3 can be determined in homogenates of kidney tissue as well as in a portion of the day 10 and 11 embryos. In a litter of embryos born to an informative mating, those which are Dh/Dh, Dh/+, and +/+ can be distinguished by the presence of the Pep‐3 allele known to be in coupling with the Dh gene. This technique makes it possible to identify and to study the limb malformations and other phenotypic effects of Dh during their development and before the limb deformity is visible.
- Massachusetts General Hospital United States
- Harvard University United States
Recombination, Genetic, Mice, Inbred C3H, Placenta, Congenital Abnormalities, Hindlimb, Pedigree, Mice, Inbred C57BL, Mice, Phenotype, Pregnancy, Animals, Female, Alleles, Peptide Hydrolases
Recombination, Genetic, Mice, Inbred C3H, Placenta, Congenital Abnormalities, Hindlimb, Pedigree, Mice, Inbred C57BL, Mice, Phenotype, Pregnancy, Animals, Female, Alleles, Peptide Hydrolases
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