
pmid: 40134352
Lanthanide‐doped upconversion nanoparticles (UCNPs) have recently demonstrated great promise in single‐particle imaging (SPI) due to their exceptional photostability and minimal background fluorescence. However, their limited brightness has posed a significant barrier to wider adoption in SPI applications. This review highlights recent advances in applying UCNPs for SPI, focusing on strategies to enhance their brightness and reduce quenching effects in aqueous environments. Additionally, it summarizes the latest progress in using UCNPs for single‐particle tracking and super‐resolution imaging, underscoring their potential in biomedical research. Finally, the review outlines current challenges and future directions in this field.
Optical Imaging, Nanoparticles, Humans, Lanthanoid Series Elements
Optical Imaging, Nanoparticles, Humans, Lanthanoid Series Elements
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