In Vitro Translation
Copyright policy )In Vitro Translation
AbstractThe two most frequently used systems for in vitro translation are the rabbit reticulocyte system and the wheat germ extract. These systems are useful for mRNAs isolated from cells, tissues, and capped or uncapped mRNA produced in vitro by transcription of cDNA. In a combined system, mRNA can be transcribed and translated in a single reaction. In addition these systems can be used for translation reactions with biotinylated amino acids; this allows capture of the newly synthesized protein using streptavidin immobilized on agarose.
- University of Maryland Marlene and Stewart Greenebaum Cancer Center United States
- National Institute of Health Pakistan
- Promega United States
Reticulocytes, Cell-Free System, Transcription, Genetic, Plant Extracts, In Vitro Techniques, Animals, Humans, Female, RNA, Messenger, Rabbits, Molecular Biology, Triticum
Reticulocytes, Cell-Free System, Transcription, Genetic, Plant Extracts, In Vitro Techniques, Animals, Humans, Female, RNA, Messenger, Rabbits, Molecular Biology, Triticum
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