Immunofluorescence Staining
Copyright policy )Immunofluorescence Staining
AbstractThis unit provides a protocol for indirect immunofluorescence, which is a method that provides information about the locations of specific molecules and the structure of the cell. Antibody molecules for a specific target molecule are exposed to the cell or tissue being investigated. The binding of these molecules is detected by incubating the sample with a secondary antibody specific for immunoglobulin molecules and conjugated to fluorophore. This provides both a visible signal and amplification of the signal and the results are observed with a fluorescent microscope.
- National Institute of Health Pakistan
- National Institutes of Health United States
Organelles, Tissue Fixation, Staining and Labeling, Fluorescent Antibody Technique, Antibodies, Specimen Handling, Antigen-Antibody Reactions, Mice, Microscopy, Fluorescence, Humans, Animals, Indicators and Reagents, Antigens, Fluorescent Antibody Technique, Indirect, Cells, Cultured, Fluorescent Dyes
Organelles, Tissue Fixation, Staining and Labeling, Fluorescent Antibody Technique, Antibodies, Specimen Handling, Antigen-Antibody Reactions, Mice, Microscopy, Fluorescence, Humans, Animals, Indicators and Reagents, Antigens, Fluorescent Antibody Technique, Indirect, Cells, Cultured, Fluorescent Dyes
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