
AbstractThis unit describes strategies for studying the proteomes of organelles, which is one example of targeted proteomics. It relies heavily on previously published units dealing with organelle preparation, protein solubilization, and proteomics techniques. A specific commentary for organelle proteomics is provided. Specific protocols for the isolation of nuclei from various sources (cell cultures, tissues) are also provided.
Organelles, Proteomics, Genomics (q-bio.GN), MESH: Cells, Molecular Biology/Genomics [q-bio.GN], 570, Cultured, MESH: Proteomics, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Cell Fractionation, FOS: Biological sciences, 616, MESH: Cell Fractionation, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Quantitative Biology - Genomics, Cells, Cultured, MESH: Organelles
Organelles, Proteomics, Genomics (q-bio.GN), MESH: Cells, Molecular Biology/Genomics [q-bio.GN], 570, Cultured, MESH: Proteomics, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Cell Fractionation, FOS: Biological sciences, 616, MESH: Cell Fractionation, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Quantitative Biology - Genomics, Cells, Cultured, MESH: Organelles
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