Controlling the spatial arrangement of optically active elements is crucial for the advancement of engineered photonic systems. Color centers in nanodiamonds (NDs) offer unique advantages for quantum sensing and information processing; however, their integration into complex optical architectures is limited by challenges in precise and reproducible positioning, as well as efficient coupling. DNA origami provides an elegant solution, as demonstrated by recent studies that showcase the nanoscale positioning of fluorescent NDs and plasmonic gold nanoparticles (NPs). A scalable and robust method is presented for covalently functionalizing NDs with DNA, enabling a high‐yield and spatially controlled assembly of diamond and gold NPs onto DNA origami. By precisely controlling the interparticle spacing, this approach reveals the distance‐dependent modulation of a nitrogen‐vacancy (NV) center photoluminescence (PL). These findings indicate selective plasmon‐driven effects. This work overcomes key limitations in current nanodiamond assembly strategies and provides insights into engineering NV PL by plasmonic coupling. These advancements bring closer to quantum photonic and sensing applications.