2‐Substituted dATP Derivatives as Building Blocks for Polymerase‐Catalyzed Synthesis of DNA Modified in the Minor Groove
Copyright policy )2‐Substituted dATP Derivatives as Building Blocks for Polymerase‐Catalyzed Synthesis of DNA Modified in the Minor Groove
Abstract2′‐Deoxyadenosine triphosphate (dATP) derivatives bearing diverse substituents (Cl, NH2, CH3, vinyl, ethynyl, and phenyl) at position 2 were prepared and tested as substrates for DNA polymerases. The 2‐phenyl‐dATP was not a substrate for DNA polymerases, but the dATPs bearing smaller substituents were good substrates in primer‐extension experiments, producing DNA substituted in the minor groove. The vinyl‐modified DNA was applied in thiol–ene addition and the ethynyl‐modified DNA was applied in a CuAAC click reaction to form DNA labelled with fluorescent dyes in the minor groove
- Charles University Czech Republic
- Institute of Organic Chemistry and Biochemistry Czech Republic
- Czech Academy of Science Czech Republic
- Academy of Sciences Library Czech Republic
- Czech Academy of Sciences Czech Republic
Base Sequence, fluorescent labelling, bioconjugation, DNA, DNA-Directed DNA Polymerase, DNA polymerase, Nucleic Acid Denaturation, nucleotides, Communications, Substrate Specificity, Deoxyadenine Nucleotides, Nucleic Acid Conformation, Thermodynamics, DNA modification
Base Sequence, fluorescent labelling, bioconjugation, DNA, DNA-Directed DNA Polymerase, DNA polymerase, Nucleic Acid Denaturation, nucleotides, Communications, Substrate Specificity, Deoxyadenine Nucleotides, Nucleic Acid Conformation, Thermodynamics, DNA modification
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